Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 7): 788-91, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23832209
ABSTRACT
Arabidopsis thaliana (At) MAP4Kalpha2, a member of the Ste20/PAK-like protein kinase family, is an essential component of the septum initiation network involved in cell division. To better understand the mode of action of AtMAP4Kalpha2, a structural biology approach has been pursued. In this study, the kinase domain of AtMAP4Kalpha2 was cloned, expressed, purified and crystallized. The crystals diffracted to 1.9 Å resolution and belonged to space group C2221, with unit-cell parameters a = 55.27, b = 82.93, c = 133.15 Å.
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MEDLINE
Assunto principal:
Proteínas Serina-Treonina Quinases
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Arabidopsis
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Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article