Brief report: miR-290-295 regulate embryonic stem cell differentiation propensities by repressing Pax6.
Stem Cells
; 31(10): 2266-72, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23843298
ABSTRACT
microRNAs of the miR-290-295 family are selectively expressed at high levels in mouse embryonic stem cells (mESCs) and have established roles in regulating self-renewal. However, the potential influence of these microRNAs on cell fate acquisition during differentiation has been overlooked. Here, we show that miR-290-295 regulate the propensity of mESCs to acquire specific fates. We generated a new miR-290-295-null mESC model, which exhibits increased propensity to generate ectoderm, at the expense of endoderm and mesoderm lineages. We further found that in wild-type cells, miR-290-295 repress Pax6 and ectoderm differentiation; accordingly, Pax6 knockdown partially rescues the mESCs differentiation impairment that is caused by loss of miR-290-295. Thus, in addition to regulating self-renewal, the large reservoir of miR-290-295 in undifferentiated mESCs fine-tunes the expression of master transcriptional factors, such as Pax6, thereby regulating the equilibrium of fate acquisition by mESC descendants.
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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MicroRNAs
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Células-Tronco Embrionárias
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article