Evaluation of a commercially available carbohydrate deficient transferrin kit to detect beta-2-transferrin in cerebrospinal fluid using capillary electrophoresis.

ABSTRACT

OBJECTIVE: The aim of this study was to evaluate the CEofix™ CDT Capillary Electrophoresis (CE) kit for the detection of ß-2-tranferrin (ß-2-Tf) in cerebrospinal fluid (CSF). DESIGN AND METHOD: Evaluation was performed according to CLSI EP5-A and EP12-A guidelines. RESULTS: The method resolved ß-2-Tf from other Tf isoforms. The C50 for ß-Tf was determined to be 0.46 mg/L. Neither hemoglobin nor bilirubin co-migrated with ß-2-Tf. CONCLUSIONS: The CDT kit can be used for detecting ß-2-Tf in CSF by CE.