Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH.
Proteins
; 81(11): 2059-63, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23852710
ABSTRACT
Conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 is a member of the aldo-keto reductase (AKR) superfamily and reduces ketopantoyl lactone to d-pantoyl lactone in a NADPH-dependent and stereospecific manner. We determined the crystal structure of CPR-C1.NADPH complex at 2.20 Å resolution. CPR-C1 adopted a triose-phosphate isomerase (TIM) barrel fold at the core of the structure in which Thr25 and Lys26 of the GXGTX motif bind uniquely to the adenosine 2'-phosphate group of NADPH. This finding provides a novel structural basis for NADPH binding of the AKR superfamily.
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Base de dados:
MEDLINE
Assunto principal:
Candida
/
Proteínas Fúngicas
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Cristalografia por Raios X
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article