Overexpression of a novel chrysanthemum Cys2/His2-type zinc finger protein gene DgZFP3 confers drought tolerance in tobacco.
Biotechnol Lett
; 35(11): 1953-9, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23881327
ABSTRACT
A drought stress-responsive Cys2/His2-type zinc finger protein gene DgZFP3 was previously isolated (Liu et al., Afr J Biotechnol 117781-7788, 2012b) from chrysanthemum. To assess roles of DgZFP3 in plant drought stress responses, we performed gain-of-function experiment. The DgZFP3-overexpression tobacco plants showed significant drought tolerance over the wild type (WT). The transgenic lines exhibited less accumulation of H2O2 under drought stress, more accumulation of proline and greater activities of peroxidase (POD) and superoxide dismutase than the WT under both control conditions and drought stress. In addition, there was greater up-regulation of the ROS-related enzyme genes (NtSOD and NtPOD) and stress-related genes (NtLEA5 and NtDREB) in transgenic lines under normal or drought conditons. Thus DgZFP3 probably plays a positive regulatory role in drought stress response and has the potential to be utilized in transgenic breeding to improve drought stress tolerance in plants.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Estresse Fisiológico
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Nicotiana
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Chrysanthemum
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Dessecação
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article