Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein: a vaccine candidate for human intestinal schistosomiasis.
Hum Vaccin Immunother
; 9(11): 2342-50, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23899507
ABSTRACT
A novel recombinant protein vaccine for human schistosomiasis caused by Schistosoma mansoni is under development. The Sm-TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. Here, we describe the cloning and the expression of the external loop of Sm-TSP-2 recombinant protein secreted by Pichia Pink the process development at 20L scale fermentation, and the two-steps purification, which resulted in a protein recovery yield of 31% and a protein purity of 97%. The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies.
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Base de dados:
MEDLINE
Assunto principal:
Esquistossomose mansoni
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Vacinas
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Proteínas de Helminto
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Tetraspaninas
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Antígenos de Helmintos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article