[Study on ultrasonic extraction and macroporous resin purification techniques of total flavones from Sedum lineare].

OBJECTIVE: To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin. METHODS: Optimum ultrasonic extraction conditions (ethanol concentration, solid/liquid, ultrasonic time, working temperature) were obtained through orthogonal design. The adsorption ratio and eluting ratio of total flavones were selected as indexes. RESULTS: The optimum ultrasonic extraction condition was 80% ethanol, solid/liquid 1:40, ultrasonic 20 min at 30 degrees C. The adsorbed AB-8 macroporous resin column was eluted by 80% ethanol at the eluting velocity of 2 mL/min, the eluting ratio and purity of total flavones was 94.72% and 43.51%, respectively. CONCLUSION: The method is simple and low cost.