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A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.
Schmitt, Sabine; Saathoff, Friederike; Meissner, Lilja; Schropp, Eva-Maria; Lichtmannegger, Josef; Schulz, Sabine; Eberhagen, Carola; Borchard, Sabine; Aichler, Michaela; Adamski, Jerzy; Plesnila, Nikolaus; Rothenfusser, Simon; Kroemer, Guido; Zischka, Hans.
Afiliação
  • Schmitt S; Institute of Molecular Toxicology and Pharmacology, Helmholtz Center Munich, German Research Center for Environmental Health, D-85764 Neuherberg, Germany.
Anal Biochem ; 443(1): 66-74, 2013 Dec 01.
Article em En | MEDLINE | ID: mdl-23969012
Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Hepatócitos / Neurônios Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Hepatócitos / Neurônios Idioma: En Ano de publicação: 2013 Tipo de documento: Article