Zn2+/Cd2+ optical discrimination by fluorescent chemosensors based on 8-hydroxyquinoline derivatives and sulfur-containing macrocyclic units.

ABSTRACT

Four new fluorescent chemosensors for metal ions based on 8-hydroxyquinoline (8-HDQ) derivatives and sulfur-containing macrocyclic units were synthesized and characterized, namely 1-(5-chloro-8-hydroxy-7-quinolinylmethyl)-1-aza-4,7,10-trithiacyclododecane (L1), 1-(5-chloro-8-hydroxy-7-quinolinylmethyl)-1-aza-4,13-dithia-7,10-dioxacyclopentadecane (L2), 1-(8-hydroxy-2-quinolinylmethyl)-1-aza-4,7,10-trithiacyclododecane (L3), and 1-(8-hydroxy-2-quinolinylmethyl)-1-aza-4,13-dithia-7,10-dioxacyclopentadecane (L4). Preliminary fluorimetric titrations indicated L1 as the only member of the family of ligands to give a selective CHEF-type response to the presence of Zn(2+) in MeCN-H2O (11, v/v) solutions, which allowed imaging of this metal ion in Cos-7 cells in vitro. The other ligands either did not show any fluorescence response (L3, L4) to any of the metal ions considered (Cu(2+), Zn(2+), Cd(2+), Hg(2+) and Pb(2+)) or gave (L2) a CHEF-type response also to the presence of Cd(2+). The coordination properties of L1 towards Zn(2+) were, therefore, fully investigated by potentiometric measurements and absorption and emission spectroscopy at different pH values, which indicated that the formation of 21 L1/Zn(2+) complexes is responsible for the CHEF-type effect observed. The complexes [Zn(L1)2H2O](BF4)2 and [Zn(L3)](ClO4)2 were characterized in the solid state by X-ray crystallography, and DFT calculations were performed to understand the origin of the Zn(2+)/Cd(2+) optical discrimination of the 8-HDQ-based "conjugate" fluorescent chemosensors reported.