ß-Arrestin 2 mediates G protein-coupled receptor 43 signals to nuclear factor-κB.
Biol Pharm Bull
; 36(11): 1754-9, 2013.
Article
em En
| MEDLINE
| ID: mdl-23985900
ABSTRACT
G-protein coupled receptor 43 (GPR43) serves as a receptor for short-chain fatty acids (SCFAs), implicated in neutrophil migration and inflammatory cytokine production. However, the intracellular signaling pathway mediating GPR43 signaling remains unclear. Here, we show that ß-arrestin 2 mediates the internalization of GPR43 by agonist. Agonism of GPR43 reduced the phosphorylation and nuclear translocation of nuclear factor-κB (NF-κB), which was relieved by short interfering RNA (siRNA) of ß-arrestin 2. Subsequently, mRNA expression of proinflammatory cytokines, interleukin (IL)-6 and IL-1ß, was downregulated by activation of GPR43 and knockdown of ß-arrestin 2 recovered the expression of the cytokines. Taken together, these results suggest that GPR43 may be a plausible target for a variety of inflammatory diseases.
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Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
/
Receptores de Superfície Celular
/
Arrestinas
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article