Generation of high-spin iron(I) in a protein environment using cryoreduction.
Inorg Chem
; 52(13): 7323-5, 2013 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-24004284
ABSTRACT
High-spin Fe(1+) sites are potentially important in iron-sulfur proteins but are rare in synthetic compounds and unknown in metalloproteins. Here, we demonstrate a spectroscopically characterized example of high-spin non-heme Fe(1+) in a protein environment. Cryoreduction of Fe(2+)-substituted azurin at 77 K with (60)Co γ radiation generates a new species with a S = (3)/2 (high-spin) Fe(1+) center having D > 0 and E/D ~ 0.25. This transient species is stable in a glycerol-water glass only up to ~170 K. A combination of electron paramagnetic resonance and Mössbauer spectroscopies provides a powerful means of identifying a transient high-spin Fe(1+) site in a protein scaffold.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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Azurina
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Proteínas de Bactérias
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Ferro
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article