In vitro selection of BNA (LNA) aptamers.
Artif DNA PNA XNA
; 4(2): 39-48, 2013.
Article
em En
| MEDLINE
| ID: mdl-24044051
ABSTRACT
Recently, we achieved the first in vitro selection of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers. High-affinity thrombin-binding aptamers (TBAs) were obtained from DNA-based libraries containing 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) in the 5'-primer region, using the method of capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). Furthermore, a similar selection protocol could provide TBAs that contain B/L nucleotides in both primer and random regions. We review technical challenges involved in the generation of various BNA libraries using analogs of B/L nucleoside-5'-triphosphate and polymerase variants and also discuss applications of these libraries to the selection of BNA (LNA) aptamers, as well as future prospects for their therapeutic and diagnostic uses.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article