Synergistic antimicrobial profiling of violacein with commercial antibiotics against pathogenic micro-organisms.
Pharm Biol
; 52(1): 86-90, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24073823
ABSTRACT
CONTEXT Chromobacterium violaceum Bergonzini (Neisseriaceae), a Gram-negative bacterium, secretes a spectacular pigment called violacein. Violacein is a quorum-sensing metabolite and is also an active antimicrobial, anticancer agent. However, its efficiency as a potential drug, alone or in synergy with other active principles, has not being completely exploited. With the advent of different multi-drug resistant strains, it becomes essential to find a new natural product(s) that could be effectively used as a therapeutic agent. OBJECTIVE:
This work focused on the extraction of violacein from an isolated strain of C. violaceum and determined the combinatory effect of violacein with commercial antibiotics against various pathogens. MATERIALS ANDMETHODS:
Violacein production was optimized and was later extracted using ethanol and characterized by liquid chromatography-mass spectrometry and infrared spectroscopy. Then, individual minimum inhibitory concentration (MIC) values for each of the antibiotics were determined followed by violacein-commercial antibiotics (11) combinations, tested at different concentrations starting from 500 to 1 µg/ml against major pathogens. RESULTS ANDDISCUSSION:
The individual MIC data for violacein was found to be 5.7 µg/ml (Staphylococcus aureus), 15.6 µg/ml (Klebsiella pneumoniae), 18.5 µg/ml (Pseudomonas aeruginosa), 20.0 µg/ml (Vibrio cholerae) and 5.7 µg/ml (Salmonella typhi). Violacein-gentamicin and violacein-cefadroxil combinations had MIC of 1.0 µg/ml against S. aureus. Most violacein-macrolide and violacein--aminoglycoside class combinations revealed fractional inhibitory concentration indices (FICI) of <0.5, thus exhibiting synergism. Furthermore, violacein-azithromycin and violacein-kanamycin combination, exhibited significant synergy (FICI-0.3) against S. typhi.CONCLUSION:
Violacein works synergistically with most commercial antibiotics and could be used as drug in combination with other antimicrobial agents.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
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Chromobacterium
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Indóis
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Antibacterianos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article