Tumor suppressor candidate TUSC3 expression during rat testis maturation.
Biosci Biotechnol Biochem
; 77(10): 2019-24, 2013.
Article
em En
| MEDLINE
| ID: mdl-24096664
ABSTRACT
Analysis of microarray data obtained by comparing gene expression between 2-week-old infant and 7-week-old mature SD rat testes revealed novel targets involved in tumor suppression. Reverse-transcription polymerase chain reaction and Northern blotting indicated that Tusc3 gene expression was upregulated in the normal maturing testis and prostate and other organs such as the cerebrum and ovary. Tumor suppressor candidate 3 protein expression was detected in these same organs at a size of about 40 kDa, in accord with the predicted molecular size. In situ hybridization and immunohistochemistry showed that mRNA and protein localization were prevalent in the testis spermatocytes and interstitial cells such as the Leydig cells, as well as prostate epithelial cells. These data suggest that TUSC3 is deeply involved in spermatogenesis in the testis, inducing sperm differentiation and maturation, and plays a role in normal prostate development and tumor suppression.
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Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Regulação da Expressão Gênica no Desenvolvimento
/
Proteínas de Transporte de Cátions
/
Proteínas Supressoras de Tumor
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article