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Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin.
Fujita, Naonobu; Morita, Eiji; Itoh, Takashi; Tanaka, Atsushi; Nakaoka, Megumi; Osada, Yuki; Umemoto, Tetsuo; Saitoh, Tatsuya; Nakatogawa, Hitoshi; Kobayashi, Shouhei; Haraguchi, Tokuko; Guan, Jun-Lin; Iwai, Kazuhiro; Tokunaga, Fuminori; Saito, Kazunobu; Ishibashi, Koutaro; Akira, Shizuo; Fukuda, Mitsunori; Noda, Takeshi; Yoshimori, Tamotsu.
Afiliação
  • Fujita N; Department of Genetics, Graduate School of Medicine, 2 Laboratory of Intracellular Membrane Dynamics, Graduate school of Frontier Biosciences, 3 Department of Molecular Virology, Research Institute for Microbial Diseases, 4 Department of Host Defense, WPI Immunology Frontier Research Center, 5 Department of Host Defense, Research Institute for Microbial Disease, and 6 Core Instrumentation Facility, Research Institute for Microbial Disease, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871,
J Cell Biol ; 203(1): 115-28, 2013 Oct 14.
Article em En | MEDLINE | ID: mdl-24100292
ABSTRACT
Although ubiquitin is thought to be important for the autophagic sequestration of invading bacteria (also called xenophagy), its precise role remains largely enigmatic. Here we determined how ubiquitin is involved in this process. After invasion, ubiquitin is conjugated to host cellular proteins in endosomes that contain Salmonella or transfection reagent-coated latex (polystyrene) beads, which mimic invading bacteria. Ubiquitin is recognized by the autophagic machinery independently of the LC3-ubiquitin interaction through adaptor proteins, including a direct interaction between ubiquitin and Atg16L1. To ensure that invading pathogens are captured and degraded, Atg16L1 targeting is secured by two backup systems that anchor Atg16L1 to ubiquitin-decorated endosomes. Thus, we reveal that ubiquitin is a pivotal molecule that connects bacteria-containing endosomes with the autophagic machinery upstream of LC3.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endossomos / Salmonella typhimurium / Autofagia / Ubiquitina Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endossomos / Salmonella typhimurium / Autofagia / Ubiquitina Idioma: En Ano de publicação: 2013 Tipo de documento: Article