Triplex real-time polymerase chain reaction assay for detection and quantification of norovirus (GI and GII) and sapovirus.
Microbiol Immunol
; 58(1): 68-71, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24117901
ABSTRACT
To improve detection of norovirus (NoVGI, NoVGII) and sapovirus (SaV), a simultaneous quantitative RT-PCR method was established. This triplex real-time PCR method was evaluated using a combination of optimized specific primers and probes. The performance of the developed PCR assay was equivalent to that of monoplex real-time PCR across a broad dynamic range of 10(2) -10(7) copies/assay using plasmid DNA standards. The limit of detection was 10(2) copies/assay. The quantitative value was comparable with that of monoplex real-time PCR of stool samples. Our triplex real-time PCR is useful for detection of NoV and SaV infections.
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Base de dados:
MEDLINE
Assunto principal:
Norovirus
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Sapovirus
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Reação em Cadeia da Polimerase Multiplex
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Reação em Cadeia da Polimerase em Tempo Real
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article