Detection of Vibrio anguillarum antigen by the dot blot assay.
J Wildl Dis
; 21(3): 211-8, 1985 Jul.
Article
em En
| MEDLINE
| ID: mdl-2411956
ABSTRACT
The dot blot assay, modified and adapted for detection of antigens from Vibrio anguillarum in fish tissues, was specific for V. anguillarum and did not react with antigens of V. ordalii, Pseudomonas sp., or Yersinia ruckeri. The blot assay enabled detection of as little as 2.3 ng of a mixture of protein antigens obtained from cell-free extracts of V. anguillarum; it was about 100 times more sensitive than either the indirect fluorescent antibody technique or bacterial isolation for detecting V. anguillarum in fish tissues.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Vibrio
/
Vibrioses
/
Doenças dos Peixes
/
Antígenos de Bactérias
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article