Building quantitative, three-dimensional atlases of gene expression and morphology at cellular resolution.
Wiley Interdiscip Rev Dev Biol
; 2(6): 767-79, 2013.
Article
em En
| MEDLINE
| ID: mdl-24123936
ABSTRACT
Animals comprise dynamic three-dimensional arrays of cells that express gene products in intricate spatial and temporal patterns that determine cellular differentiation and morphogenesis. A rigorous understanding of these developmental processes requires automated methods that quantitatively record and analyze complex morphologies and their associated patterns of gene expression at cellular resolution. Here we summarize light microscopy-based approaches to establish permanent, quantitative datasets-atlases-that record this information. We focus on experiments that capture data for whole embryos or large areas of tissue in three dimensions, often at multiple time points. We compare and contrast the advantages and limitations of different methods and highlight some of the discoveries made. We emphasize the need for interdisciplinary collaborations and integrated experimental pipelines that link sample preparation, image acquisition, image analysis, database design, visualization, and quantitative analysis.
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MEDLINE
Assunto principal:
Atlas como Assunto
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Blastoderma
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Regulação da Expressão Gênica no Desenvolvimento
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Imageamento Tridimensional
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Drosophila melanogaster
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Células Eucarióticas
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article