Characterization of N-demethyllincosamide methyltransferases LmbJ and CcbJ.

Najmanová, Lucie; Kutejová, Eva; Kadlec, Jan; Polan, Marek; Olsovská, Jana; Benada, Oldrich; Novotná, Jitka; Kameník, Zdenek; Halada, Petr; Bauer, Jacob; Janata, Jirí

Najmanová, Lucie; Kutejová, Eva; Kadlec, Jan; Polan, Marek; Olsovská, Jana; Benada, Oldrich; Novotná, Jitka; Kameník, Zdenek; Halada, Petr; Bauer, Jacob; Janata, Jirí.

Afiliação

Najmanová L; Institute of Microbiology AS CR, Vídenská 1083, 142 20, Prague 4 (Czech Republic).

Chembiochem ; 14(17): 2259-62, 2013 Nov 25.

Article em En | MEDLINE | ID: mdl-24166757

ABSTRACT

ABSTRACT

Chemical diversity Two SAM-dependent N-methyltransferases-LmbJ from the biosynthesis of the antibiotic lincomycin and CcbJ from celesticetin biosynthesis-have been characterized and compared. Both tested enzymes form multimers and are able to utilize N-demethyllincomycin, the natural substrate of LmbJ, with comparable efficiency.

Assuntos

Antibacterianos/biossíntese; Biocatálise; Lincomicina/biossíntese; Lincosamidas/biossíntese; Metiltransferases/metabolismo; Antibacterianos/química; Lincomicina/química; Lincosamidas/química; Metiltransferases/química; Conformação Molecular; Especificidade por Substrato

Palavras-chave

N-methyltransferases; Streptomyces; antibiotics; lincomycin; natural products

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Lincomicina / Lincosamidas / Biocatálise / Metiltransferases / Antibacterianos Idioma: En Ano de publicação: 2013 Tipo de documento: Article

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