Ectopic expression of transcription factor AP-2δ in developing retina: effect on PSA-NCAM and axon routing.
J Neurochem
; 129(1): 72-84, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24188130
ABSTRACT
Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address the role of AP-2δ in retina by in ovo electroporation of RCAS/AP-2δ retroviral constructs into the eyes of chick embryos at day 2 of gestation. Ectopic expression of AP-2δ does not affect lineage differentiation in the developing retina. However, immunostaining of retinal tissue with markers associated with axonal growth such as growth-associated protein 43 and polysialic acid-neural cell adhesion molecule (PSA-NCAM) demonstrates axonal misrouting and abnormal axonal bundling. Treatment of AP-2δ-misexpressing retinal cell cultures with endoneuraminidase, an enzyme that removes PSA from NCAM, decreases AP-2δ-induced axonal bundling. Our data suggest a role for AP-2δ in polysialylation of NCAM, with ectopic expression of AP-2δ resulting in premature bundling of emerging axons and misrouting of axons. We propose that expression of AP-2δ in a subset of ganglion cells contributes to the fine-tuning of axonal growth in the developing retina.
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Assunto principal:
Retina
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Ácidos Siálicos
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Axônios
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Coristoma
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Regulação da Expressão Gênica no Desenvolvimento
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Molécula L1 de Adesão de Célula Nervosa
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Fator de Transcrição AP-2
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article