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Analysis of acidic endogenous phytohormones in grapes by using online solid-phase extraction coupled with LC-MS/MS.
Yu, Jian-Na; Meng, Qing-Yan; Liu, Wen-Jie; Lu, Ya-Ling; Ren, Xiao-Lin.
Afiliação
  • Yu JN; College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China College of Life Science, Tarim University, Xinjiang 843300, China.
  • Meng QY; College of Life Science, Tarim University, Xinjiang 843300, China.
  • Liu WJ; College of Life Science, Tarim University, Xinjiang 843300, China.
  • Lu YL; College of Life Science, Tarim University, Xinjiang 843300, China.
  • Ren XL; College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China renxl@nwsuaf.edu.cn.
J Chromatogr Sci ; 52(9): 1145-9, 2014 Oct.
Article em En | MEDLINE | ID: mdl-24200641
ABSTRACT
Phytohormones play important roles in regulating numerous plant physiological and developmental processes, even during the postharvest storage period. In order to determine the functions and changes of gibberellins acid (GA3), indoleacetic acid (IAA), abscisic acid (ABA), indolebutyric acid (IBA) and jasmonic acid (JA) in grape berries during storage, an ultrasensitive method based on direct injection online solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. Grape berries were extracted with cold methanol. After centrifugation, the supernatants were concentrated with a vacuum centrifugal concentrator and injected into an online solid-phase extraction column. After the cleanup procedure, the analytes were determined by LC-MS/MS. The results showed that the linearity of the proposed method was 10-210 µg kg(-1) for ABA, 20-200 µg kg(-1) for IBA, 15-320 µg kg(-1) for IAA, 20-320 µg kg(-1) for GA3 and 3.0-90.0 µg kg(-1) for JA. The limits of detection of the method were 0.71, 2.79, 0.94, 0.39 and 0.57 µg kg(-1), respectively. The proposed method was successfully applied to the analysis of endogenous phytohormones in grape berries during the postharvest storage period.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reguladores de Crescimento de Plantas / Cromatografia Líquida de Alta Pressão / Vitis / Espectrometria de Massas em Tandem / Extração em Fase Sólida Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reguladores de Crescimento de Plantas / Cromatografia Líquida de Alta Pressão / Vitis / Espectrometria de Massas em Tandem / Extração em Fase Sólida Idioma: En Ano de publicação: 2014 Tipo de documento: Article