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Molecular detection and partial characterization of Tomato Yellow Leaf Curl Virus in Sri Lanka.
Samarakoon, S A M C; Balasuriya, A; Rajapaksha, R G A S; Wickramarachchi, W A R T.
Afiliação
  • Samarakoon SA; Faculty of Agriculture, Rajarata University of Sri Lanka, Puliyankulama, Anuradhapura, Sri Lanka.
Pak J Biol Sci ; 15(18): 863-70, 2012 Sep 15.
Article em En | MEDLINE | ID: mdl-24205755
Tomato Yellow Leaf Curl Virus (TYLCV) is an important plant virus on one of the economically most important vegetable crops; tomato (Lycopersicon esculentum Mill.). This had not been molecularly detected before, in Sri Lanka. TYLCV-GN-SL was isolated from apparently infected tomato plants using modified Cetyltrimethyl Ammonium Bromide (CTAB) method in Gannoruwa. Associated Begomoviruses were detected using Deng 541/Deng 540 and AV 494/AC 1048 primer pairs. TYLCV was detected for the first time in tomato in Sri Lanka using P1V/P4C, TYLCV specific primer pair. Nucleotide sequence of coat protein of isolated TYLCV-GN-SL proved that the Indian strain of ToLC virus was closely related to Tomato Leaf Curl Sri Lanka Virus (TLCV-SL: 97%) and Tomato leaf curl Geminivirus (TLCGV: 93%) through direct sequencing data. TLCV-SL was confirmed as TYLCV isolate. TYLCV was molecularly detected from major tomato growing districts like Badulla, Nuwara-Eliya, Kandy and Matale in Sri Lanka.
Assuntos
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Base de dados: MEDLINE Assunto principal: Solanum lycopersicum / Begomovirus Idioma: En Ano de publicação: 2012 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Solanum lycopersicum / Begomovirus Idioma: En Ano de publicação: 2012 Tipo de documento: Article