Adiabatic Rotor-Echo-Short-Pulse-Irradiation mediated cross-polarization.

ABSTRACT

We present a new dipolar recoupling method for efficient and robust heteronuclear polarization transfer in solid-state NMR under magic-angle-spinning (MAS) conditions. The method combines the recent (RESPIRATION)CP method with a modulation of the amplitude of the rotor-synchronized pulses at one of the involved rf channels through the recoupling condition. In this manner, it is possible to achieve high transfer efficiencies while maintaining robustness towards rf-field inhomogeneities and resonance offsets. The performance of the so-called adiabatic-(RESPIRATION)CP experiment is demonstrated numerically and experimentally using uniformly (13)C,(15)N-labeled samples of alanine and ubiquitin. In particular for cases with relatively high rf inhomogeneity, the scheme offers advantages over the commonly used dipolar recoupling pulse sequences.