Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells.
FEBS Lett
; 588(1): 15-20, 2014 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-24239544
ABSTRACT
The Rab-GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta-cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta-cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta-cells and whether it plays, as AS160/TBC1D4, a role in beta-cell function and survival. Using human and rat beta-cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta-cells resulted in increased basal and glucose-stimulated insulin release, decreased proliferation but no change in apoptosis.
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Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Proteínas Ativadoras de GTPase
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Células Secretoras de Insulina
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Insulina
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article