Intranuclear microinjection for transformation of tomato callus cells.
Plant Cell Rep
; 7(5): 293-6, 1988 Aug.
Article
em En
| MEDLINE
| ID: mdl-24241867
ABSTRACT
An efficient method, called the culture plate method, was devised for microinjection of foreign materials into nuclei of tomato callus cells. The culture plate method, used in this study, is advantageous because cells suitable for microinjection can be selected microscopically and the injected cells subsequently cultured in the same plate. With this microinjection system, some foreign materials were injected into nuclei of callus cells without causing detrimental effects. Kanamycin-resistant callus clones were obtained 1 month after injection from single cells whose nuclei were microinjected with a NPT II DNA fragment of the pE2KX plasmid.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article