Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record.
Infect Genet Evol
; 21: 214-9, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24270016
ABSTRACT
In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8(∗) region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.
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MEDLINE
Assunto principal:
Infecções por Rotavirus
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Surtos de Doenças
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Rotavirus
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Proteínas do Capsídeo
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Gastroenterite
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Antígenos Virais
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article