Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 12): 1335-8, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24316824
ABSTRACT
Here, a 1.82â
Å resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Actinomycetales
/
Modelos Moleculares
/
Glicosídeo Hidrolases
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article