Monitoring G protein-coupled receptor activation using an adenovirus-based ß-arrestin bimolecular fluorescence complementation assay.

Song, Yong Bhum; Park, Chul O; Jeong, Jae-Yeon; Huh, Won-Ki

Song, Yong Bhum; Park, Chul O; Jeong, Jae-Yeon; Huh, Won-Ki.

Afiliação

Song YB; Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul 151-747, Republic of Korea.
Park CO; Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul 151-747, Republic of Korea.
Jeong JY; Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, Ansan 426-744, Republic of Korea. Electronic address: jeongjy@kiost.ac.
Huh WK; Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul 151-747, Republic of Korea; Institute of Microbiology, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: wkh@snu.ac.kr.

Anal Biochem ; 449: 32-41, 2014 Mar 15.

Article em En | MEDLINE | ID: mdl-24361713

Assuntos

Adenoviridae/genética; Arrestinas/metabolismo; Técnicas Biossensoriais/métodos; Receptores Acoplados a Proteínas G/metabolismo; Clonagem Molecular; DNA Complementar/genética; Fluorescência; Células HEK293; Ensaios de Triagem em Larga Escala/métodos; Humanos; Receptores Acoplados a Proteínas G/genética; beta-Arrestinas

Palavras-chave

Adenovirus; Bimolecular fluorescence complementation (BiFC); G protein-coupled receptor (GPCR); High-throughput screening; ß-Arrestin

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Adenoviridae / Arrestinas / Receptores Acoplados a Proteínas G Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google