A new protocol to detect multiple foodborne pathogens with PCR dipstick DNA chromatography after a six-hour enrichment culture in a broad-range food pathogen enrichment broth.

Hayashi, Masahiro; Natori, Tatsuya; Kubota-Hayashi, Sayoko; Miyata, Machiko; Ohkusu, Kiyofumi; Kawamoto, Keiko; Kurazono, Hisao; Makino, Souichi; Ezaki, Takayuki

Hayashi, Masahiro; Natori, Tatsuya; Kubota-Hayashi, Sayoko; Miyata, Machiko; Ohkusu, Kiyofumi; Kawamoto, Keiko; Kurazono, Hisao; Makino, Souichi; Ezaki, Takayuki.

Afiliação

Hayashi M; Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan ; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Natori T; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Kubota-Hayashi S; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Miyata M; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Ohkusu K; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Kawamoto K; Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Kurazono H; Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Makino S; Department of Domestic Science, Kyoto Seibo College, Kyoto 612-0878, Japan.
Ezaki T; Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.

Biomed Res Int ; 2013: 295050, 2013.

Article em En | MEDLINE | ID: mdl-24364031

Assuntos

DNA Bacteriano/isolamento & purificação; Microbiologia de Alimentos; Doenças Transmitidas por Alimentos/genética; Contagem de Colônia Microbiana; DNA Bacteriano/genética; Escherichia coli/genética; Escherichia coli/isolamento & purificação; Escherichia coli/patogenicidade; Doenças Transmitidas por Alimentos/microbiologia; Humanos; Reação em Cadeia da Polimerase; Salmonella enterica/genética; Salmonella enterica/isolamento & purificação; Salmonella enterica/patogenicidade; Shigella/genética; Shigella/isolamento & purificação; Shigella/patogenicidade

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Bacteriano / Microbiologia de Alimentos / Doenças Transmitidas por Alimentos Idioma: En Ano de publicação: 2013 Tipo de documento: Article

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