Phototoxic effects of lysosome-associated genetically encoded photosensitizer KillerRed.
J Biomed Opt
; 19(7): 071403, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24365992
ABSTRACT
KillerRed is a unique phototoxic red fluorescent protein that can be used to induce local oxidative stress by green-orange light illumination. Here we studied phototoxicity of KillerRed targeted to cytoplasmic surface of lysosomes via fusion with Rab7, a small GTPase that is known to be attached to membranes of late endosomes and lysosomes. It was found that lysosome-associated KillerRed ensures efficient light-induced cell death similar to previously reported mitochondria- and plasma membrane-localized KillerRed. Inhibitory analysis demonstrated that lysosomal cathepsins play an important role in the manifestation of KillerRed-Rab7 phototoxicity. Time-lapse monitoring of cell morphology, membrane integrity, and nuclei shape allowed us to conclude that KillerRed-Rab7-mediated cell death occurs via necrosis at high light intensity or via apoptosis at lower light intensity. Potentially, KillerRed-Rab7 can be used as an optogenetic tool to direct target cell populations to either apoptosis or necrosis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fármacos Fotossensibilizantes
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Apoptose
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Proteínas de Fluorescência Verde
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Lisossomos
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Necrose
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article