Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4.
World J Microbiol Biotechnol
; 10(1): 33-5, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-24420882
ABSTRACT
A thermophilic Bacillus stearothermophilus strain AP-4 excreting a thermostable alkaline protease, was isolated from a local compost. Maximum activity of protease (250 U/ml) was after 36 h growth in broth at pH 9.0 and at 55°C. The protease was optimally active at pH 9.0 and 55°C and was stable in 5 mM CaCl2. The enzyme was completely inactivated by PMSF, EDTA and ß-mercaptoethanol. It is therefore a metal ion-dependent, alkaline, serine protease.
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MEDLINE
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En
Ano de publicação:
1994
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Article