Sample preparation for single virion atomic force microscopy and super-resolution fluorescence imaging.
J Vis Exp
; (83): e51366, 2014 Jan 02.
Article
em En
| MEDLINE
| ID: mdl-24429535
ABSTRACT
Immobilization of virions to glass surfaces is a critical step in single virion imaging. Here we present a technique adopted from single molecule imaging assays which allows adhesion of single virions to glass surfaces with specificity. This preparation is based on grafting the surface of the glass with a mixture of PLL-g-PEG and PLL-g-PEG-Biotin, adding a layer of avidin, and finally creating virion anchors through attachment of biotinylated virus specific antibodies. We have applied this technique across a range of experiments including atomic force microscopy (AFM) and super-resolution fluorescence imaging. This sample preparation method results in a control adhesion of the virions to the surface.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Virologia
/
Microscopia de Força Atômica
/
Imagem Óptica
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article