Analysis of microglial migration by a micropipette assay.
Nat Protoc
; 9(2): 491-500, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24481275
Microglial cells have important roles in maintaining brain homeostasis, and they are implicated in multiple brain diseases. There is currently interest in investigating microglial migration that results in cell accumulation at focal sites of injury. Here we describe a protocol for rapidly triggering and monitoring microglial migration by using a micropipette assay. This protocol is an adaptation of the axon turning assay using microglial cells. Chemoattractants released from the micropipette tip produce a chemotactic gradient that induces robust microglial migration. In combination with microscopic imaging, this assay allows simultaneous recording of cell movement and subcellular compartment trafficking, along with quantitative analysis. The actual handling time for the assay takes â¼2-3 h in total. The protocol is simple, inexpensive and convenient to set up, and it can be adopted to examine cell migration in multiple cell types, including cancer cells with a wide range of chemical signals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Encefalopatias
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Transdução de Sinais
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Movimento Celular
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Microglia
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Ensaios de Migração Celular
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article