A simple chemical synthesis of sugar nucleoside diphosphates in water.
Curr Protoc Nucleic Acid Chem
; 54: 13.12.1-13.12.10, 2013 Oct 08.
Article
em En
| MEDLINE
| ID: mdl-24510796
ABSTRACT
Chemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection-deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio- and stereospecificity, but also the glycosyl donor to provide the sugar that is added. This unit describes a simple synthesis of sugar-nucleoside diphosphates (sugar-NDPs), the type of glycosyl donor (e.g., UDP-Glc, UDP-Gal, ADP-Glc) required by most glycosyltransferases, by using a chemical coupling reaction in water. The preparation of sugar-NDPs by this method therefore does not require any skills in synthetic organic chemistry.
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Base de dados:
MEDLINE
Assunto principal:
Glicosiltransferases
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Técnicas de Química Sintética
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Açúcares de Nucleosídeo Difosfato
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article