[Influence of zoledronate on osteoclast differentiation and gene expression of calmodulin and calmodulin-dependent protein kinase II].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 48(11): 694-8, 2013 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-24513077
ABSTRACT
OBJECTIVE:
To investigate the effect of zoledronate acid on osteoclast differentiation and gene expression of calmodulin (CAM) and calmodulin-dependent protein kinase (CAMK)II.METHODS:
Receptor activation of nuclear factor κB ligand (RANKL) was used to induce differentiation of RAW264.7 cells into osteoclasts in vitro. The cells were divided into two groups, group A and group B. Both groups were treated with RANKL for 5 days, whereas group B was also treated with zoledronate for the last 2 days.Osteoclastogenesis and gene expression of CAM and CAMK II were examined.RESULTS:
In group B, the number of new-generated osteoclasts (≥3 nuclei), number and size of dentin resorption lacunaes were (23 ± 3) , (19 ± 2) and (4951 ± 223) µm(2) respectively, which were significantly lower than those [(44 ± 3) , (46 ± 1) and (13 331 ± 248) µm(2)] in group A (P < 0.01).mRNA and protein level of CAM and CAMK II were also significantly down-regulated in group B when compared with group A (P < 0.01) and the decrease was 26.7% and 37.2% respectively for CAM, 57.0% and 76.1% respectively for CAMK II.CONCLUSIONS:
Zoledronate acid could significantly inhibit formation and resorption function of osteoclasts. CAM and CAMKII may be involved in the inhibition process.
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Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Calmodulina
/
Diferenciação Celular
/
Difosfonatos
/
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina
/
Imidazóis
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article