Flux analysis in plant metabolic networks: increasing throughput and coverage.
Curr Opin Biotechnol
; 26: 183-8, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24561560
ABSTRACT
Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analysis or determined experimentally by (13)C-Metabolic Flux Analysis. These relatively complicated and time-consuming methods have recently seen significant improvements at the level of coverage and throughput. Metabolic models have developed from single cell models into whole-organism dynamic models. Advances in lab automation and data handling have significantly increased the throughput of flux measurements. This review summarizes advances to increase coverage and throughput of metabolic flux analysis in plants.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Redes e Vias Metabólicas
/
Análise do Fluxo Metabólico
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article