Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
; 159(3): 388-93, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-24566377
ABSTRACT
BACKGROUND:
This study was aimed to investigate whether osteoblasts from diabetic patients have a promoting effect on osteogenesis of human umbilical cord mesenchymal stem cells (HUMSCs).METHODS:
HUMSCs were co-cultured with osteoblasts of diabetic and non-diabetic patients. Morphological appearance and cytochemical characteristics of the non-diabetic osteoblasts and diabetic osteoblasts were observed by hematoxylin-eosin staining, type I collagen protein expression, alkaline phosphatase (ALP) staining and Alizarin Red S staining. Cell viability, type I collagen protein expression, ALP activity and osteocalcin mRNA expression in HUMSCs were investigated.RESULTS:
Compared with negative control group, the cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA were increased in HUMSCs co-cultured with diabetic and non-diabetic osteoblasts (P<0.05). There was no statistically significant difference in the HUMSCs cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA between the non-diabetic and diabetic group (P >0.05).CONCLUSIONS:
Similar to osteoblasts from non-diabetic patients, osteoblasts from diabetic patients also have the ability to promote HUMSCs proliferation, and leading to HUMSCs exhibit some characteristic of osteoblasts.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Osteogênese
/
Diabetes Mellitus
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article