Tracking of cell nuclei for assessment of in vitro uptake kinetics in ultrasound-mediated drug delivery using fibered confocal fluorescence microscopy.
Mol Imaging Biol
; 16(5): 642-51, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24566957
ABSTRACT
PURPOSE:
Previously, we demonstrated the feasibility to monitor ultrasound-mediated uptake of a cell-impermeable model drug in real time with fibered confocal fluorescence microscopy. Here, we present a complete post-processing methodology, which corrects for cell displacements, to improve the accuracy of pharmacokinetic parameter estimation. PROCEDURES Nucleus detection was performed based on the radial symmetry transform algorithm. Cell tracking used an iterative closest point approach. Pharmacokinetic parameters were calculated by fitting a two-compartment model to the time-intensity curves of individual cells.RESULTS:
Cells were tracked successfully, improving time-intensity curve accuracy and pharmacokinetic parameter estimation. With tracking, 93 % of the 370 nuclei showed a fluorescence signal variation that was well-described by a two-compartment model. In addition, parameter distributions were narrower, thus increasing precision.CONCLUSIONS:
Dedicated image analysis was implemented and enabled studying ultrasound-mediated model drug uptake kinetics in hundreds of cells per experiment, using fiber-based confocal fluorescence microscopy.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ultrassom
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Núcleo Celular
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Sistemas de Liberação de Medicamentos
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Microscopia Confocal
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Rastreamento de Células
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Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article