Hybrid capture 2 assay based evaluation of high-risk HPV status in healthy women of north-east India.
Asian Pac J Cancer Prev
; 15(2): 861-5, 2014.
Article
em En
| MEDLINE
| ID: mdl-24568508
ABSTRACT
BACKGROUND:
High risk HPV (HR-HPV) testing has been recommended as an effective tool along with cytology screening in identification of cervical intraepithelial lesions (CINs) and prevention of their progress towards invasive cervical cancer. The aim of this study was to assess the HR-HPV DNA status by Hybrid Capture 2 (HC2) assay in healthy asymptomatic women of North-East India. MATERIALS ANDMETHODS:
This study examined cervical cell samples of forty three (n=43) healthy women by HC2 assay. A High Risk HPV DNA kit (Qiagen) was used which can detect 13 high risk HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68.RESULTS:
The mean relative light units (RLU) for samples was in the range of 141-5, 94, 619. HR-HPV DNA was confirmed in 16% (7/43) of participant women samples. Among demographic and clinical parameters, menstrual irregularity (p=0.039) and infection history (p=0.028) has shown statistically significant differences between the HR-HPV-positive and negative groups. In the HR-HPV positive group, two women were confirmed for CINs after colposcopy and histopathologic examination.CONCLUSIONS:
We suggest that there may be an association between irregular menstruation and infection history of the urogenital tract with HR-HPV DNA prevalence in North-East Indian asymptomatic women. HC2 assay can be a valuable tool for HR-HPV screening.
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Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Kit de Reagentes para Diagnóstico
/
Displasia do Colo do Útero
/
Neoplasias do Colo do Útero
/
Colo do Útero
/
Infecções por Papillomavirus
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article