Establishment of an agamid cell line and isolation of adenoviruses from central bearded dragons (Pogona vitticeps).
J Vet Diagn Invest
; 26(2): 221-5, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24569225
ABSTRACT
A cell line was established from whole 6-8-week-old central bearded dragon (Pogona vitticeps) embryos. Cells were mid-sized and showed an elongated and polymorphic form. The cell line grew in a monolayer and has been serially passaged for 17 passages at time of publication. This cell line has been used with samples from adenovirus polymerase chain reaction (PCR)-positive bearded dragons, and 2 virus isolates have been obtained so far. The isolates show a clear cytopathic effect in inoculated cells. Both virus isolates have been serially passaged on this cell line, and have been identified by PCR amplification and sequencing of a portion of the DNA-dependent DNA polymerase gene and show 100% nucleotide identity to the corresponding region of an agamid adenovirus. Electron microscopic examination of supernatant from infected cells demonstrated the presence of nonenveloped particles, with a diameter of approximately 80 nm in both virus isolates.
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Base de dados:
MEDLINE
Assunto principal:
Cultura de Vírus
/
Atadenovirus
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Lagartos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article