Transposon insertional mutagenesis models of cancer.
Cold Spring Harb Protoc
; 2014(3): 235-47, 2014 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-24591685
ABSTRACT
Transposon-based insertional mutagenesis in the mouse provides a powerful approach for identifying new cancer genes. Transposon insertions in cancer genes are selected during tumor development because of their positive effect on tumor growth, and the transposon insertion sites in tumors thus serve as tags for identifying new cancer genes. Direct comparisons of transposon-mutated genes in mouse tumors with mutated genes in human tumors can lend insight into the genes and signaling pathways that drive tumorigenesis. This is critical for prioritizing genes for further study, either for their efficacy as biomarkers or drug targets. In this article, we will introduce DNA transposon-based systems used for gene discovery in mice and discuss their application to identify candidate cancer genes in light of recently published tumor studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Elementos de DNA Transponíveis
/
Mutagênese Insercional
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Neoplasias
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article