[MIP-1α promotes the migration ability of Jurkat cell through human brain microvascular endothelial cell monolayer].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 22(1): 35-9, 2014 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-24598647
ABSTRACT
This study was purposed to explore the mechanism of central nervous system (CNS) leukemia resulting from brain metastasis of human acute T-cell leukemia (T-ALL) cells and the role of MIP-1α in migration of Jurkat cells through human brain microvascular endothelial cells (HBMEC). The real-time PCR, siRNA test, transendothelial migration test, endothelial permeability assay and cell adhesion assay were used to detect MIP-1α expression, penetration and migration ability as well as adhesion capability respectively. The results showed that the MIP-1α expression in Jurkat cells was higher than that in normal T cells and CCRF-HSB2, CCRF-CEM , SUP-T1 cells. The MIP-1α secreted from Jurkat cells enhanced the ability of Jurkat cells to penetrate through HBMEC, the ability of Jurkat cells treated by MIP-1α siRNA to adhere to HBMEC and to migrate trans endothelial cells decreased. It is concluded that the MIP-1α secreted from Jurkat cells participates in process of penetrating the Jurkat cells through HBMEC monolayer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Encefálicas
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Células Endoteliais
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Leucemia-Linfoma Linfoblástico de Células T Precursoras
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Quimiocina CCL3
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article