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Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing.
Monteiro, Jussimara; Widen, Raymond H; Pignatari, Antonio C C; Kubasek, Carly; Silbert, Suzane.
Afiliação
  • Monteiro J; Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States; Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Widen RH; Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States.
  • Pignatari AC; Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Kubasek C; Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States.
  • Silbert S; Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States. Electronic address: ssilbert@tgh.org.
J Microbiol Methods ; 100: 42-5, 2014 May.
Article em En | MEDLINE | ID: mdl-24631557
ABSTRACT
A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Variação Genética / Beta-Lactamases / Análise de Sequência de DNA / Klebsiella pneumoniae Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Variação Genética / Beta-Lactamases / Análise de Sequência de DNA / Klebsiella pneumoniae Idioma: En Ano de publicação: 2014 Tipo de documento: Article