Omics technologies and their applications to evaluate metal toxicity in mice M. spretus as a bioindicator.
J Proteomics
; 104: 4-23, 2014 Jun 02.
Article
em En
| MEDLINE
| ID: mdl-24631825
ABSTRACT
Metals are important components of living organisms since many biological functions critically depend on their interaction with some metal in the cell. However, human activities have increased toxic metal levels in the terrestrial and aquatic ecosystems affecting living organisms. The impact of metals on cellular metabolism and global homeostasis has been traditionally assessed in free-living organisms by using conventional biomarkers; however, to obtain a global vision of metal toxicity mechanisms and the responses that metals elicit in the organisms, new analytical methodologies are needed. We review the use of omics approaches to assess the response of living organisms under metal stress illustrating the possibilities of different methodologies on the basis of our previous results. Most of this research has been based on free-living mice Mus spretus, a conventional bioindicator used to monitor metal pollution in Doñana National Park (DNP) (SW Spain), which is an important European biological reserve for migrating birds affected by agricultural, mining and industrial activities. The benefits of using omic techniques such as heterologous microarrays, proteomics methodologies (2-DE, iTRAQ®), metallomics, ionomics or metabolomics has been remarked; however, the complexity of these areas requires the integration of omics to achieve a comprehensive assessment of their environmental status. This article is part of a Special Issue entitled Environmental and structural proteomics. BIOLOGICAL SIGNIFICANCE:
This work presents new contributions in the study of environmental metal pollution in terrestrial ecosystems using Mus spretus mice as bioindicator in Doñana National Park (SW Spain) and surroundings. In addition, it has been demonstrated that the integration of omics multi-analytical approaches provides a very suitable approach for the study of the biological response and metal interactions in exposed and free-living mice (Mus musculus and Mus spretus, respectively) under metal pollution.Palavras-chave
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MEDLINE
Assunto principal:
Bioensaio
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Regulação da Expressão Gênica
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Proteômica
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Metaboloma
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Transcriptoma
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Metais
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Testes de Mutagenicidade
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article