Tributyltin induces cell cycle arrest at G1 phase in the yeast Saccharomyces cerevisiae.
J Toxicol Sci
; 39(2): 311-7, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24646713
Tributyltin (TBT) has long been recognized as a major environmental pollutant that can cause significant damage to the cellular functions as well as disruption of endocrine homeostasis. TBT induces apoptosis accompanied by production of reactive oxygen species (ROS) in mammalian and yeast cells. We observed that the budding yeast cells exposed to this compound at low concentrations exhibited cell growth arrest, but not cell death. Flow cytometric analysis of yeast cells without synchronization and morphological assessment of cells synchronized at M phase by nocodazole treatment indicated that TBT-exposed Saccharomyces cerevisiae cells were arrested at G1 phase of the cell cycle. This arrest was recovered by the addition of N-acetylcysteine, suggesting the involvement of ROS production by TBT. This is the first study to evaluate the action of TBT on cell cycle events.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Compostos de Trialquitina
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Fase G1
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Espécies Reativas de Oxigênio
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Poluentes Ambientais
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Pontos de Checagem do Ciclo Celular
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article