Development of a serology-based assay for efficacy evaluation of a lactococcicosis vaccine in Seriola fish.
Fish Shellfish Immunol
; 38(1): 135-9, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24657319
ABSTRACT
Lactococcicosis is an infection caused by the bacterium Lactococcus garvieae and creates serious economic damage to cultured marine and fresh water fish industries. The use of the assay currently applied to evaluate the potency of the lactococcicosis vaccine is contingent upon meeting specific parameters after statistical analysis of the percent survival of the vaccinated yellowtail or greater amberjack fish after challenge with a virulent strain of L. garvieae. We found that measuring the serological response with a quantitative agglutinating antibody against the L. garvieae antigen (phenotype KG+) was an effective method of monitoring the potency of lactococcicosis vaccines. Vaccinated fish had significantly higher antibody titers than control fish when the L. garvieae Lg2-S strain was used as an antigen. Furthermore, the titer of the KG + agglutinating antibody was correlated with vaccine potency, and the cut-off titer was determined by comparing the data with those from the challenge test. An advantage of the proposed serology-based potency assay is that it will contribute to reduced numbers of animal deaths during vaccine potency evaluations.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vacinas Bacterianas
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Lactococcus
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Infecções por Bactérias Gram-Positivas
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Doenças dos Peixes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article