Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin-streptavidin detection system.

Ahmed, Raju; Spikings, Emma; Zhou, Shaobo; Thompsett, Andrew; Zhang, Tiantian

Ahmed, Raju; Spikings, Emma; Zhou, Shaobo; Thompsett, Andrew; Zhang, Tiantian.

Afiliação

Ahmed R; Institute of Biomedical and Environmental Sciences and Technology (iBEST), University of Bedfordshire, 250 Butterfield, Great Marlings, Luton, Bedfordshire LU2 8DL, UK. Electronic address: raju79@hotmail.co.uk.
Spikings E; Institute of Biomedical and Environmental Sciences and Technology (iBEST), University of Bedfordshire, 250 Butterfield, Great Marlings, Luton, Bedfordshire LU2 8DL, UK.
Zhou S; Institute of Biomedical and Environmental Sciences and Technology (iBEST), University of Bedfordshire, 250 Butterfield, Great Marlings, Luton, Bedfordshire LU2 8DL, UK.
Thompsett A; School of Health, Sport and Bioscience, University of East London, Stratford Campus, London E15 4LZ, UK.
Zhang T; School of Applied Sciences, Bournemouth University, Poole, Dorset BH12 5BB, UK.

J Immunol Methods ; 406: 143-7, 2014 Apr.

Article em En | MEDLINE | ID: mdl-24657589

Assuntos

Biotina/imunologia; Western Blotting/métodos; Estreptavidina/imunologia; Peixe-Zebra/embriologia; Actinas/imunologia; Animais; Anticorpos/imunologia; Biotinilação/métodos; Humanos

Palavras-chave

Biotinylated secondary antibody; Endogenous biotin binding; Non-specific bands; Qdot 625 streptavidin conjugate; Streptavidin; Western blot

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biotina / Peixe-Zebra / Western Blotting / Estreptavidina Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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