Reversible re-programing of cell-cell interactions.
Angew Chem Int Ed Engl
; 53(20): 5112-6, 2014 May 12.
Article
em En
| MEDLINE
| ID: mdl-24700601
ABSTRACT
The ability to engineer and re-program the surfaces of cells would provide an enabling synthetic biological method for the design of cell- and tissue-based therapies. A new cell surface-engineering strategy is described that uses lipid-chemically self-assembled nanorings (lipid-CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non-toxic FDA-approved drug, the nanorings were quickly disassembled and the cell-cell interactions reversed. Similar to T-cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti-EpCAM-lipid-CSANs, they were shown to selectively kill antigen-positive cancer cells. Taken together, these results demonstrate that lipid-CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell-cell interactions.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Comunicação Celular
/
Reprogramação Celular
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article