Comparison of 2 synthetically generated recombinant prions.
Prion
; 8(2)2014.
Article
em En
| MEDLINE
| ID: mdl-24721728
ABSTRACT
Prion is a protein-conformation-based infectious agent causing fatal neurodegenerative diseases in humans and animals. Our previous studies revealed that in the presence of cofactors, infectious prions can be synthetically generated in vitro with bacterially expressed recombinant prion protein (PrP). Once initiated, the recombinant prion is able to propagate indefinitely via serial protein misfolding cyclic amplification (sPMCA). In this study, we compared 2 separately initiated recombinant prions. Our results showed that these 2 recombinant prions had distinct biochemical properties and caused different patterns of spongiosis and PrP deposition in inoculated mice. Our findings indicate that various recombinant prions can be initiated in vitro and potential reasons for this variability are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article