The 14-day repeated dose liver micronucleus test with methapyrilene hydrochloride using young adult rats.
Mutat Res Genet Toxicol Environ Mutagen
; 780-781: 123-7, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-24768639
ABSTRACT
The repeated dose liver micronucleus (RDLMN) assay using young adult rats has the potential to detect genotoxic hepatocarcinogens that can be integrated into a general toxicity study. The assay methods were thoroughly validated by 19 Japanese facilities. Methapyrilene hydrochloride (MP), known to be a non-genotoxic hepatocarcinogen, was examined in the present study. MP was dosed orally at 10, 30 and 100mg/kg/day to 6-week-old male CrlCD (SD) rats daily for 14 days. Treatment with MP resulted in an increase in micronucleated hepatocytes (MNHEPs) with a dosage of only 100mg/kg/day. At this dose level, cytotoxicity followed by regenerative cell growth was noted in the liver. These findings suggest that MP may induce clastogenic effects indirectly on the liver or hepatotoxicity of MP followed by regeneration may cause increase in spontaneous incidence of MNHEPs.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carcinógenos
/
Testes para Micronúcleos
/
Hepatócitos
/
Fígado
/
Metaqualona
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article